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Updated: Jul 17, 2026

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活细胞对酶基质相互作用的成像显示了PTP1BB的空间调节

Ivan A Yudushkin1, Andreas Schleifenbaum, Ali Kinkhabwala

  • 1European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Science (New York, N.Y.)
|January 6, 2007
PubMed
概括

蛋白氨酸酸酶1B (PTP1B) 的活性在细胞内受到空间调节,形成一个梯度,允许受体氨酸激酶 (RTK) 信号和终止. 这种空间控制对于管理细胞生长信号至关重要.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...