循环 permuted 转移RNA 的折叠
T Pan1, R R Gutell, O C Uhlenbeck
1Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Science (New York, N.Y.)
|November 29, 1991
概括
研究人员在酵母转移RNA (tRNA) 中确定了所有可切割的核糖酸盐链接,而不会影响分子折叠. 这项研究揭示了tRNA折叠图案可以在其他RNA序列中内部出现.
科学领域:
- 分子生物学分子生物学
- RNA结构和功能 RNA结构和功能
- 生物化学 生物化学
背景情况:
- 转移RNA (tRNA) 分子具有复杂的三维结构,对它们的生物功能至关重要.
- 了解对维持tRNA折叠至关重要的特定联系对于破译RNA结构-功能关系至关重要.
研究的目的:
- 确定酵母tRNA中的所有可在不破坏分子整体折叠的情况下分裂的酸盐-核糖链接.
- 调查tRNA折叠基因在其他RNA序列中的流行率和潜在的内部发生.
主要方法:
- 在酵母tRNA(Phe) 上使用循环顺序分析.
- 分子经历了有限的性水解,以引入单个随机断裂.
- 正确折叠的tRNA被识别在中性pH下使用分离,这是一个已知的tRNA正确折叠的指标.
主要成果:
- 在单个实验中生成了可切割的核糖酸盐链接的全面地图.
- 令人惊的是,大多数循环 permuted tRNA 分子保持了正确的折叠.
- 随后的计算机搜索在Genbank中的其他RNA序列中发现了许多类似折叠模式的实例.
结论:
- 该研究成功地绘制了酵母tRNA中的labile链接,提供了对其结构完整性的见解.
- 这些发现表明,特征性的tRNA折叠模式可以作为各种RNA分子的内部结构元素.
- 这一发现为了解RNA折叠原理开辟了超出正规tRNA结构的新途径.
相关概念视频
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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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