波罗将一个PICH送到kinetochore中
1Fox Chase Cancer Center, Philadelphia, PA 19083, USA. timothy.yen@fccc.edu
Cell
|January 16, 2007
概括
一个新发现的螺旋酶,PICH,与分离的姐妹动态细胞之间的线索结合. 建议PICH通过监测中心体DNA张力来调节线粒检查点.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 姐妹动态细胞在相期间分离.
- 线性检查点确保了适当的染色体分离.
- 运动 - 微管附着物对于细胞分裂至关重要.
研究的目的:
- 确定参与监测中心基DNA的蛋白质.
- 研究PICH在线粒检查点中的作用.
- 阐明检查点调节的机制在中位数.
主要方法:
- 免疫光显微镜可视化PICH局部化.
- 生物化学测试用于研究蛋白质相互作用.
- 在细胞中分析了变化的PICH水平的线粒性进展.
主要成果:
- PICH (KIF2C) 局部化到连接分离的姐妹动态核的线程.
- 这些线程被认为是链接的中心基因DNA.
- PICH将Mad2招募到kinetochores中,从而为线粒检查点做出贡献.
- 建议PICH通过感知中介质张力来调节检查点信号.
结论:
- PICH在线粒检查点中起着至关重要的作用.
- PICH 在中位数上充当张力传感器.
- 了解PICH功能可以了解染色体分离的忠实性.
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