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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Measuring the Kinetics of mRNA Transcription in Single Living Cells
11:22

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Published on: August 25, 2011

一种系统方法来测量转录因子的约束能量场景.

Sebastian J Maerkl1, Stephen R Quake

  • 1Biochemistry and Molecular Biophysics Option, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|January 16, 2007
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种微流体平台,用于测量分子相互作用亲和力,这对于预测生物网络功能至关重要. 该平台成功地描述了转录因子DNA结合的特征,有助于预测它们的体内作用.

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

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Last Updated: Jul 7, 2026

Measuring the Kinetics of mRNA Transcription in Single Living Cells
11:22

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Published on: August 25, 2011

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

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科学领域:

  • 系统生物学 系统生物学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 预测生物网络功能是系统生物学的一个关键目标.
  • 定量参数,特别是分子相互作用亲和力,往往是未知的.
  • 短暂和低亲和相互作用的高通量测量具有挑战性.

研究的目的:

  • 开发一种高通量方法来测量分子相互作用亲和力.
  • 描述真核细胞转录因子的DNA结合能量景观.
  • 利用这些景观来测试具有约束力的假设和预测体内功能.

主要方法:

  • 开发一个高通量微流体平台.
  • 利用分子相互作用的机械捕获来进行亲和度测量.
  • 对四种真核细胞转录因子的DNA结合能量景观的表征.

主要成果:

  • 成功实施了微流体平台,用于高通量亲和度测量.
  • 对于选定的转录因子,DNA结合能量景观的详细表征.
  • 结合景观在预测体内转录因子功能的证明有用性.

结论:

  • 微流体平台可有效测量分子相互作用特性.
  • 有特征的结合景观为转录因子行为提供了洞察力.
  • 这种方法推进了对生物网络功能的预测.