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在未折叠的多链中,端到端与内部循环形成的动力学.
1Division of Biophysical Chemistry, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Journal of the American Chemical Society
|January 18, 2007
概括
对于循环类型来说,蛋白质折叠动力学不同. 内部循环 (II型和III型) 的形成速度比端到端循环 (I型) 慢,原因是内部灵活性降低,影响蛋白质结构的形成.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 物理化学 物理化学
背景情况:
- 蛋白质折叠涉及分子内相互作用和循环形成.
- 之前的研究集中在终端循环形成上,但内部接触至关重要.
- 了解循环形成动力学是蛋白质折叠机制的关键.
研究的目的:
- 为了比较蛋白质折叠过程中不同循环形成类型的动力学.
- 为了研究循环位置 (端到端,端到内部,内部到内部) 对折叠率的影响.
- 阐明内部链的灵活性和溶剂相互作用在循环形成中的作用.
主要方法:
- 利用三倍三倍的能量转移 (从香到纳氨酸) 来测量循环形成动力学.
- 类型I (端到端),类型II (端到内部) 和类型III (内部到内部) 循环的比较动力学.
- 分析了循环大小,氨基酸序列和链维度对速率常数的影响.
主要成果:
- 类型II和类型III的循环形成比类型I的类似大小和序列的循环形成要慢得多.
- 类型II循环形成率随着链条尺寸的增加而降低,变得比类型I慢约2.5倍.
- 第三种类型的循环形成速度比第二种类型慢约1.7倍,表明连接链运动.
结论:
- 循环形成动力学的差异主要归因于内部多链灵活性的变化.
- 溶剂相互作用影响循环形成,但不能解释循环类型之间的观察到的动力差异.
- 内部链的灵活性决定了不同环位置的独特折叠动态.
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