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多种有机小分子的合成和细胞分析

Annaliese K Franz1, Philip D Dreyfuss, Stuart L Schreiber

  • 1Department of Chemistry and Chemical Biology, Harvard University, Howard Hughes Medical Institute, Chemical Biology Program, Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA.

Journal of the American Chemical Society
|February 1, 2007
PubMed
概括

No abstract available in PubMed .

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...