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相关概念视频

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...

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相关实验视频

Updated: Jul 17, 2026

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10:44

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Published on: May 15, 2019

修改后的DNA胺体,可以结合高反选择性thalidomide衍生物的 (R) 异构体.

Atsushi Shoji1, Masayasu Kuwahara, Hiroaki Ozaki

  • 1Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515 Japan.

Journal of the American Chemical Society
|February 1, 2007
PubMed
概括

研究人员开发了一种经过修改的DNA吸收体,可以选择性地与 (R) - thalidomide结合. 这种新型的体,是通过指数式丰富的连接体的系统演化而创建的,为thalidomide enantiomer分析提供了更好的稳定性和结合亲和力.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 化学生物学 化学生物学

背景情况:

  • thalidomide enantiomers表现出明显的生物活性,因此需要使用enantioselective分析工具.
  • 与天然DNA相比,开发修改后的DNA体可以增强结合亲和力和稳定性.

研究的目的:

  • 创建和描述一种能够对enantioselectivethalidomide识别的新型DNA吸收体.
  • 调查thalidomide结合的结构基础和修饰DNA基的作用.

主要方法:

  • 通过指数式丰富 (SELEX) 的连接体的系统演化,使用修改后的DNA库.
  • 表面等离子体共振 (SPR) 和光定位用于结合分析.
  • 用于结构预测的计算序列分析.

主要成果:

  • 选择了一种经过修改的DNA胺体,该胺体特别与 (R) - thalidomide enantioselectively结合.
  • 修改后的基功能组对于thalidomide的结合是必不可少的.
  • 一个截断的阿帕特马体在发针凸起区域内确定了结合部位, (R) - 塔利多米德衍生物的Kd为1.0μM.

更多相关视频

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

相关实验视频

Last Updated: Jul 17, 2026

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10:44

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Published on: May 15, 2019

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

结论:

  • 开发的修改后的DNA体是用于识别thalidomide的高度选择性工具.
  • 这种阿普坦可以用于生物化学分析和研究thalidomide enantiomers的生物作用.
  • 这些发现突出了改性核酸在开发选择性分子探针方面的潜力.