双基RNA局部化需要对内体的分类复合体进行特定的结合
Uwe Irion1, Daniel St Johnston
1Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
Nature
|February 3, 2007
概括
该ESCRT-II复合体,特别是VPS36,直接结合双形mRNA的定位信号,使得Staufen-依赖的运输到Drosophila胚胎的前部进行正常发育.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 双胞胎mRNA定位到Drosophila胚胎的前部对于通过双胞胎蛋白质梯度进行头-胸部模式至关重要.
- 除了Staufen之外,mRNA运输的精确机制和所涉及的蛋白质仍然在很大程度上是未知的.
- 众所周知,ESCRT-II复合体用于内体排序,但其在mRNA局部化中的作用尚不清楚.
研究的目的:
- 阐明双形mRNA被运送到Drosophila卵子细胞前部的机制.
- 为了识别与双mRNA局部化信号结合的特定蛋白质.
- 研究ESCRT-II复合体在mRNA本地化过程中的作用.
主要方法:
- 对缺乏ESCRT-II复杂子单元 (VPS22,VPS25,VPS36) 的Drosophila突变的分析.
- 通过生物化学测试,研究VPS36与双基mRNA 3' UTR之间的相互作用.
- 评估VPS36和Staufen的局部定位与双形mRNA相关.
主要成果:
- 在ESCRT-II子单元的突变取消了最终的Staufen-依赖的双mRNA定位的步骤.
- VPS36通过其GLUE域特别结合双体3' UTR的茎环V.
- VPS36在卵细胞前部的局部化依赖于双形mRNA,并且对于Staufen招募是必需的.
结论:
- 该ESCRT-II复合体,特别是VPS36,作为一个序列特定的RNA结合蛋白,用于双 mRNA定位.
- VPS36是第一个特定的蛋白质,它专门识别了双形mRNA定位信号.
- 这种ESCRT-II的RNA结合功能得到保护,并阐明了其非内体的角色.
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