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在细胞周期晚期所需的新酵母活性蛋白样基因.

E Schwob¹, R P Martin

¹Laboratoire de Biochimie, Centre National de la Recherche Scientifique, Strasbourg, France.

|January 9, 1992

概括

此摘要是机器生成的。

研究人员发现了ACT2,这是一种新型酵母基因,它编码了一种类似actin的蛋白质,对细胞生长至关重要. 这种蛋白质与47%的actin相同,在细胞骨重组中发挥作用,突出了真核生物中actin类蛋白质的保存功能.

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科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

动氨酸是一种高度保守的细胞骨蛋白质,对真核细胞的功能如运动和分裂至关重要.
酵母菌Saccharomyces cerevisiae有一个必不可少的活性蛋白基因 (ACT1),对细胞组织和极化生长至关重要.
之前的研究集中在高度保守的actin蛋白,对不同的actin样分子的知识有限.

研究的目的:

为了描述新发现的酵母基因ACT2及其编码的蛋白质.
研究Act2蛋白的功能和结构特性.
探索真核生物中不同类型的行为蛋白的进化意义.

主要方法:

在Saccharomyces cerevisiae中鉴定和描述ACT2的基因.
蛋白质序列分析和与已知actin结构进行比较.
在已知的3D结构上,Act2的结构叠加.
对酵母菌植物生长的ACT2基因要求的评估.

主要成果:

发现ACT2,一种酵母分裂基因,编码391氨基酸蛋白 (Act2),与酵母actin相同47%.
Act2蛋白在聚合和结合关键的循环中表现出显著的分歧,而核心域仍然保留.

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ACT2对于酵母细胞的植物生长至关重要.

其他真核生物中也存在类似actin类蛋白质的相关基因,这表明它们的功能保留了下来.

结论:

该Act2蛋白代表了第一个描述的高度分离的actin分子.
结构和生理学数据表明,Act2在细胞循环期间的细胞骨重组中起作用.
发现ACT2及其保存的同类突显了除了规范性actin之外的actin类蛋白质的重要性.