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相关概念视频

Protein Folding01:22

Protein Folding

Overview
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
DNA Packaging00:58

DNA Packaging

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Protein Folding01:22

Protein Folding

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DNA Packaging00:58

DNA Packaging

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Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...

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相关实验视频

Updated: Jul 15, 2026

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
06:37

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

Published on: February 9, 2010

在细胞中蛋白质折叠.

M J Gething1, J Sambrook

  • 1Department of Biochemistry, University of Texas Southwestern Medical Centre, Dallas 75235.

Nature
|January 2, 1992
PubMed
概括

在体内蛋白质折叠依赖于氨基酸序列和保存的辅助蛋白质. 虽然一些蛋白质单独折叠,但细胞组装涉及进化稳定的宏分子成分.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 蛋白质构成是由氨基酸序列决定的.
  • 对于一些孤立的蛋白质,体外再折叠是可能的.
  • 在体内蛋白质的折叠和组装涉及细胞组件.

研究的目的:

  • 突出细胞组件在蛋白质折叠中的作用.
  • 强调保护蛋白在多组合中的参与.
  • 为了对比体外和体外蛋白质折叠机制.

主要方法:

  • 关于蛋白质折叠机制的文献综述.
  • 对参与组装的保存蛋白家族的分析.
  • 在体外和体外蛋白质折叠研究的比较.

主要成果:

  • 在体内蛋白质折叠和组装需要辅助蛋白质.
  • 许多参与细胞折叠的蛋白质在物种之间高度保存.
  • 细胞环境和宏分子组件对于正确的折叠至关重要.

结论:

  • 氨基酸序列决定了最终的蛋白质构成,但细胞机械对于体内折叠和组装至关重要.

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06:37

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4D Imaging of Protein Aggregation in Live Cells
08:59

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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

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  • 保存的蛋白质在确保细胞内适当的蛋白质结构和功能方面发挥着至关重要的作用.
  • 在体内折叠的理解为细胞蛋白质稳定和疾病机制提供了洞察力.