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Updated: May 1, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
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在酵母染色体生物学中涉及的蛋白质复合物的功能剖析,使用遗传相互作用地图

Sean R Collins1, Kyle M Miller, Nancy L Maas

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158, USA.

Nature
|February 23, 2007
PubMed
概括

这项研究引入了Epistatic Miniarray Profile (E-MAP) 来绘制743个酵母基因之间的遗传相互作用. E-MAP数据揭示了蛋白质复合体内的功能关系,并将它们组织成通路,有助于了解细胞生物学.

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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae07:48

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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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