一个切片介导的机制,用于重复关联siRNA 5'端形成在Drosophila
Lalith S Gunawardane1, Kuniaki Saito, Kazumichi M Nishida
1Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan.
概括
在Drosophila中,重复关联的小干扰RNA (rasiRNAs) 是静止逆转移子的关键. 来自感官链的AGO3关联 rasiRNAs通过互补相互作用引导PIWI介导的沉默.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 德罗斯菲拉菌种系通过Dicer独立的途径产生重复关联的小干扰RNA (rasiRNA).
- PIWI亚家族的阿尔戈纳特,包括茄子 (Aub),Piwi和AGO3,通过使用rasiRNAs来调解逆转移素沉默.
研究的目的:
- 描述与PIWI亚家族成员AGO3.3相关的小RNA.
- 阐明 rasiRNA 生物发生和功能在 Drosophila 生殖系沉默中的机制.
主要方法:
- 与AGO3.3相关的小RNA的测序.
- 对AGO3,Aub和Piwi相关的 rasiRNA进行比较分析.
- 对于Aub和AGO的体外切片活性测试3.
主要成果:
- 与AGO3相关的 rasiRNAs主要来源于反转换子的感觉链,与Aub和Piwi相关的 rasiRNAs (反感觉) 不同.
- AGO3 rasiRNAs在核酸10处表现出对腺因的偏好,而Aub/Piwi rasiRNAs则在5'端偏好 uracil.
- 在AGO3-和Aub相关的 rasiRNAs的5'端之间观察到互补性.
- 在体外,Aub和AGO3都表现出了切片活性.
结论:
- 提出了一个模型,其中链偏差的 rasiRNA 生产和 PIWI Slicer 活动协调以指导 rasiRNA 前体处理.
- 这种机制确保了Drosophila生殖系中可转移元素的有效沉默.
相关概念视频
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...


