线粒体电子运输在血液阶段的特定作用 菌 Plasmodium falciparum
Heather J Painter1, Joanne M Morrisey, Michael W Mather
1Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA.
Nature
|March 3, 2007
概括
疟疾寄生虫 (Plasmodium falciparum) 中的线粒体对于胺合成至关重要. 取代一个酶使它们对电子传输抑制剂产生了耐药性,这表明这种途径的作用并不重要.
科学领域:
- 线粒体生物学 线粒体生物学
- 寄生虫学的寄生虫学
- 简介:真核生物的进化过程.
背景情况:
- 线粒体起源于内共生 prokaryotes,并在eukaryotes中经历了显著的遗传变化.
- 虫寄生虫表现出高度减少的线粒体基因组,有些人甚至完全缺乏它们.
- 疟疾寄生虫Plasmodium falciparum在其红细胞阶段具有独特的线粒体作用.
研究的目的:
- 在Plasmodium falciparum的红细胞阶段研究线粒体电子运输链的特定功能.
- 确定是否针对这种途径可以成为开发抗疟疾药物的可行策略.
主要方法:
- 产生了转基因的Plasmodium falciparum寄生虫,表达了来自Saccharomyces cerevisiae的修饰的二基酸脱酶酶.
- 在线粒体电子运输链抑制剂的存在下评估了寄生虫存活率和药物敏感性.
- 测量了对药物治疗的反应中的线粒体膜潜力.
主要成果:
- 杆菌的线粒体电子运输链主要是必不可少的,用于皮里米丁生物合成的乌比金再生.
- 表达替代酶的转基因寄生虫对线粒体电子传输抑制剂有抗性.
- 寄生虫的生存能力仍然取决于线粒体膜潜力,对proguanil表现出过敏.
结论:
- 红细胞Plasmodium falciparum中的线粒体电子运输链,如果金胺生物合成与ubiquinone再生脱,则无需使用.
- 针对特定的代谢功能,而不是整个电子运输链,可能为抗疟疾疗法提供新的途径.
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