对酵母复制原始激活时间的位置影响
1Department of Genetics, University of Washington, Seattle 98195.
Cell
|January 24, 1992
概括
细胞DNA复制时间由一个时间程序调节. 在酵母体中,移动复制起源表明,它们的染色体位置,特别是靠近端粒的位置,会影响它们在S阶段激活时.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 细胞染色体复制是一种马赛克过程,具有早期和晚期复制的DNA区域.
- 这种时间模式表明DNA复制起源的编程激活.
- 了解复制起源时间的调节对于细胞周期控制至关重要.
研究的目的:
- 研究酵母 (Saccharomyces cerevisiae) 中DNA复制起源的时间调节的基础.
- 为了确定控制原始激活时间的 cis 作用元件是否与原始函数的决定因素可分离.
- 探索染色体位置在决定复制时间方面的作用.
主要方法:
- 两个酵母复制起源的染色体位置,ARS1 (早期激活) 和ARS501 (晚期激活),被改变了.
- 分析了这些重新定位的起源的功能影响和激活时间.
主要成果:
- 控制复制原始激活时间的 cis 作用信息可以与原始功能必不可少的元素脱.
- 发现ARS501起源的晚期激活受其与端粒的近距离的影响.
结论:
- 染色体背景,特别是端粒的接近,在DNA复制起源激活的时间调节中起着重要作用.
- 复制时间受到内在起源属性和外在染色体定位的影响.
相关概念视频
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