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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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一个光传感器用于成像活细胞中的可逆回氧循环

Evan W Miller1, Shelly X Bian, Christopher J Chang

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal of the American Chemical Society
|March 6, 2007
PubMed
概括

No abstract available in PubMed .

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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