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相关概念视频

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium08:45

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Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper...
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Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their...
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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization08:16

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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent...
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This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.
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Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ...
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Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental...
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相关实验视频

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老龄化,脂肪和卡路里限制.

Luigi Fontana1, Samuel Klein

  • 1Division of Geriatrics and Nutritional Science and Center for Human Nutrition, Washington University School of Medicine, St Louis, Mo 63110, USA. lfontana@im.wustl.edu

JAMA
|March 8, 2007
PubMed
概括
此摘要是机器生成的。

成年人的卡路里限制显示了与动物相似的代谢益处,但人类寿命的最佳摄入量仍然未知. 过度限制可以导致营养不良和不良影响.

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科学领域:

  • 老年学是一门学科.
  • 营养科学 营养科学
  • 代谢健康 代谢健康

背景情况:

  • 由于过度摄入卡路里而导致的肥胖会增加慢性疾病的风险,并减少预期寿命.
  • 在动物中,摄入足够营养的卡路里限制 (CR) 可以延长寿命并降低疾病风险.

研究的目的:

  • 评估卡路里限制与足够的营养摄入量的生理和临床影响.

主要方法:

  • 在PubMed,老年病学期刊和参考清单上进行了全面的文献搜索.
  • 包括流行病学,病例控制和随机对照试验.
  • 基于同行评审,样本大小,客观性和偏见最小化,对审查报告的质量评估.

主要成果:

  • 成年人类的CR诱导了与CR动物和子相似的代谢,激素和炎症适应.
  • 这些适应包括降低糖尿病,心血管疾病和潜在的癌症风险因素.
  • 过度限制卡路里导致营养不良和不良的临床结果.

结论:

  • 成年人的卡路里限制促进有益的代谢,荷尔蒙和功能变化.
  • 人类健康和寿命的最佳卡路里摄入量和身体脂肪尚未确定.
  • 适度CR可能对特定人群构成风险,例如瘦肉个体.