在芽酵母中复制独立的基因素周转率的动力学
Michael F Dion1, Tommy Kaplan, Minkyu Kim
1Faculty of Arts and Sciences, Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
Science (New York, N.Y.)
|March 10, 2007
概括
酵母中的质子周转显示了动态色素. 促进者和边界的快速替换表明了分离功能基因组区域的机制.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 染色体动态对于在各种时间尺度上运行的细胞过程至关重要.
- 了解基质子周转,可以了解染色体的调节和功能.
研究的目的:
- 通过测量活体酵母细胞中素H3的周转率来研究染色质的动态行为.
- 为了确定基因组不同区域 (如促进体和编码区域) 的基因组替代率是如何变化的.
主要方法:
- 利用基因组板阵列进行高分辨率测量素H3周转率.
- 在G1被捕的Saccharomyces cerevisiae中,在基因组的4%的单核分辨率和整个基因组的较低分辨率下测试的周转率.
主要成果:
- 与编码区域相比,核细胞替代率在促进区域显著高于编码区域.
- 编码区域中的质子周转率与聚合酶密度正相关.
- 在已知的染色体边界元素中观察到快速的基因组循环.
结论:
- 快速的基因素循环在分离不同的染色质域中起着功能性作用.
- 这种动态过程可以防止基因组修饰和状态在相邻的基因组区域之间异常传播.
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