回溯以因子依赖的方式确定RNAP II的力敏感性
Eric A Galburt1, Stephan W Grill, Anna Wiedmann
1Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Nature
|March 16, 2007
概括
RNA聚合酶II (RNAP II) 的回溯限制了转录力. 转录因子TFIIS拯救了回溯的RNAP II,使转录能够在真核细胞中抵抗更高的力量.
科学领域:
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- RNA聚合酶II (RNAP II) 转录了真核细胞信使RNA,这是一个关键和受调节的细胞过程.
- RNAP II能够穿越细胞屏障的能力是基因调节的关键.
研究的目的:
- 研究来自Saccharomyces cerevisiae的单个RNAP II分子的力动力学.
- 确定转录延长因子TFIIS在RNAP II对力和逆行反应中的作用.
主要方法:
- 采用单分子双陷光学刀试验.
- 采用了一种新的方法来为活性RNAP II复合体进行丰富.
- 在TFIIS的存在和缺席下研究RNAP II转录动态.
主要成果:
- 不仅仅是强迫,RNAP II的回溯也决定了转录的限制.
- RNAP II停止了转录,并且无法从~7.5 pN的回溯中恢复.
- 回溯持续时间遵循t(-3/2) 功率定律,暗示离散基对扩散.
- TFIIS挽救了回溯的RNAP II,允许转录高达16.9 pN.
结论:
- 像TFIIS这样的真核转录因子可以调节RNAP II的机械性能.
- TFIIS增强了RNAP II克服回溯和转录更高力量的能力.
- 这突出了转录延长在真核生物中的调节机制.
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