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相关概念视频

Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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General Transcription Factors01:30

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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T Cell Types and Functions

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Na&#239;ve CD4+ T Cells Using a TGF-&#946;-containing Protocol
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Foxp3通过与AML1/Runx1相互作用来控制调节性T细胞功能.

Masahiro Ono1, Hiroko Yaguchi, Naganari Ohkura

  • 1Department of Experimental Pathology, Institute for Frontier Medical Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

Nature
|March 23, 2007
PubMed
概括

调节性T细胞 (T) 维持免疫平衡. 转录因子Foxp3与AML1相互作用,抑制IL-2和IFN-,控制T(R) 细胞功能和免疫反应.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 转录因子功能的作用

背景情况:

  • 自然产生的CD25+CD4+调节性T细胞 (T(R细胞) 对于维持免疫自我耐受性和免疫平衡至关重要.
  • T (R) 细胞抑制异常免疫反应,防止自身免疫性疾病和过敏.
  • 转录因子Foxp3是T(R) 细胞发育和功能的关键调节者,其子宫外表达赋予抑制活性.

研究的目的:

  • 阐明Foxp3控制T(R) 细胞功能的分子机制,包括细胞因子的产生和T(R) 细胞相关分子的表达.
  • 研究转录因子AML1 (急性髓性白血病1) /Runx1在调节T(R) 细胞相关基因表达中的作用.
  • 探索准Foxp3-AML1相互作用以控制T细胞介导免疫反应的潜力.

主要方法:

  • 研究了Foxp3和AML1在自然T(R) 细胞中的相互作用.
  • 分析了这种相互作用对IL-2和IFN-gamma的基因表达的影响.
  • 评估了对T(R) 细胞相关分子的上调和整体抑制活性的影响.

主要成果:

  • 证明AML1/Runx1激活了常规CD4+T细胞中的IL-2和IFN-玛基因表达.
  • 表明Foxp3在自然T(R) 细胞中与AML1进行物理相互作用.
  • 提供了证据表明,这种Foxp3-AML1相互作用抑制IL-2和IFN-马生成,调节T(R) 细胞分子,并赋予抑制活性.

结论:

  • 福克斯p3和AML1之间的相互作用是控制T(R) 细胞功能和抑制活性的关键机制.
  • 这种由Foxp3和AML1进行的转录控制对于维持免疫平衡至关重要.
  • 准Foxp3-AML1相互作用为调节T细胞介导的免疫反应在生理和病理条件下提供了潜在的策略.