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相关概念视频

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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相关实验视频

Updated: May 10, 2026

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
08:40

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Published on: November 29, 2016

在G2/M过渡期间,ERK-MAP激酶对Cdc25C进行调节.

Ruoning Wang1, Guangan He, Mayra Nelman-Gonzalez

  • 1Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.

Cell
|March 27, 2007
PubMed
概括

线素激活蛋白激酶 (MAPK) 途径对于细胞周期进展至关重要. 这项研究表明,ERK-MAP激酶直接酸化并激活Cdc25酸酶,这是G(2) /M转变的关键调节者,在介质和线粒细胞周期中.

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

相关实验视频

Last Updated: May 10, 2026

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
08:40

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Published on: November 29, 2016

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • G(2) / M 阶段过渡是由蛋白质酸酶 Cdc25 调节的,它需要通过酸化来激活.
  • 虽然Cdc2/cyclin B和Polo-like kinase (PLK) 可以在体外激活Cdc25,但它们的活性不足以解释M阶段诱导期间的Cdc25激活.

研究的目的:

  • 为了研究p42MAP激酶 (MAPK) 的作用,ERK2的Xenopus ortolog,在G(2) / M过渡期间激活Cdc25.
  • 为了确定ERK-MAP激酶是否参与哺乳动物细胞中的Cdc25激活.

主要方法:

  • 在Xenopus卵和卵细胞中对蛋白质相互作用和酸化的分析.
  • 在哺乳动物细胞系中抑制ERK激活,以评估其对Cdc25C酸化和激活的影响.

主要成果:

  • p42 MAPK被确定为M相停止的Xenopus蛋提取物中主要的Cdc25酸化激酶.
  • 在Xenopus卵细胞中,p42 MAPK与Cdc25相互作用,并在介质诱导过程中在特定位置 (T48,T138,S205) 酸化它,增强其酸酶活性.
  • 在哺乳动物细胞中,ERK1/2与Cdc25C相互作用,并在线粒分裂过程中在T48酸化它. 抑制ERK激活部分损害了Cdc25C激活和线粒诱导.

结论:

  • 在G(2) /M过渡期间,ERK-MAP激酶直接参与Cdc25的激活.
  • 这种机制在不同物种中得到保护,突出了ERK-MAP激酶在细胞循环调节中的基本作用.