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相关概念视频

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

39.1K
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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相关实验视频

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

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复发性流行病的季节性动态.

Lewi Stone1, Ronen Olinky, Amit Huppert

  • 1Biomathematics Unit, Department of Zoology, Faculty of Life Science, Tel Aviv University, Ramat Aviv 69978, Israel. lewi@post.tau.ac.il

Nature
|March 30, 2007
PubMed
概括

季节性显著影响传染病的动态. 这项研究确定了一个新的门,可以预测未来的流行病爆发或流行病爆发.

科学领域:

  • 流行病学 流行病学
  • 数学生物学 数学生物学
  • 生态生态学 生态生态学

背景情况:

  • 季节性深刻影响自然系统和人口动态.
  • 传染病的传播,包括流感和麻疹,受到季节性强迫的强烈影响.
  • 了解宿主-寄生虫关系和疾病复发对于公共卫生至关重要.

研究的目的:

  • 分析季节性强迫SIR (敏感,传染性,恢复) 流行病模型的非线性动态.
  • 调查流行后的动态,并确定未来疫情的预测值.
  • 探索疫情爆发时间 (阶段) 在季节性疾病模式中的流行病学意义.

主要方法:

  • 对经典的季节性强迫SIR流行病模型的分析.
  • 专注于流行后的动态,而不是疫情爆发的开始.
  • 基于人口易感性和招聘率的新门效应的确定.

主要成果:

  • 一个新的值效应预测了流行病爆发或"跳过"年份.
  • 建立了清晰的分析条件,以预测流行病的发生.
  • 疫情爆发的时间 (阶段) 提供了有价值的流行病学信息,影响疾病的传播和强度.

更多相关视频

Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

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相关实验视频

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

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Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

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结论:

  • 季节性强迫可以通过调节疾病传播来控制流行病的触发和强度.
  • 人口易感性和招募率是未来流行病风险的关键决定因素.
  • 这些发现为新兴和重新出现的载体传播疾病提供了相关的预测工具.