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Updated: Jun 30, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Published on: November 23, 2016

几乎同质的光敏感胺基脊柱替代允许酶活性在核糖酶中切换

Dirk Wildemann1, Cordelia Schiene-Fischer, Tobias Aumüller

  • 1Max-Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, D-06120 Halle/Saale, Germany.

Journal of the American Chemical Society
|April 3, 2007
PubMed
概括

用氧结合物对蛋白质骨干进行光开关,可以控制生物活性. 修改后的RNase S在暴露于紫外线光线时显示了废除的酶活性,这是可逆的.

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...