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德洛索菲拉的学习和记忆基因鲁塔巴加编码了一个对Ca2+/卡尔莫杜林敏感的腺酸环酶

L R Levin¹, P L Han, P M Hwang

¹Howard Hughes Medical Institute, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.

|February 7, 1992

概括

果中的鲁塔巴加基因编码了一种对/卡尔莫杜林敏感的腺酸环酶,这对学习和记忆至关重要. 灭活这种酶的突变会导致学习缺陷,证实了它的作用.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

腺基环酶是细胞信号通路中的关键酶.
德洛索菲拉 (Drosophila melanogaster rutabaga) 基因与学习和记忆有关.
哺乳动物大脑的腺循环酶对和素有反应.

研究的目的:

在Drosophila melanogaster中识别和表征腺环酶基因.
为了确认鲁塔巴加基因的身份和功能.
调查Ca2+/calmodulin-responsive腺环酶在学习和记忆中的作用.

主要方法:

序列同质性分析以识别假定的腺环酶基因.
在哺乳动物系统中Drosophila rutabaga的表达.
用/calmodulin进行酶活性测定.
对rut1突变和P元素插入的遗传分析.

主要成果:

鉴定出了四个Drosophila腺酸环酶基因,包括鲁塔巴加.
在哺乳动物系统中,Ca2+/calmodulin显著刺激了鲁塔巴加循环酶活性.
在rut1中发生的突变取消了腺环酶的活性.
影响鲁塔巴加的突变映射到它的5'端.

结论:

鲁塔巴加基因位是对Ca2+/calmodulin敏感的腺酸环酶的结构基因.
这种腺环酶的无活化会导致Drosophila的学习和记忆缺陷.

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