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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: May 13, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

U5 snRNA与5'和3'拼接位的外子序列相互作用.

A J Newman1, C Norman

  • 1MRC Laboratory of Molecular Biology, Cambridge, England.

Cell
|February 21, 1992
PubMed
概括

酵母U5小核RNA (snRNA) 中的突变通过启用基配对来激活异常的mRNA前拼接. 这表明U5 snRNA.

科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 遗传学 遗传学 是一个

背景情况:

  • U5小核RNA (snRNA) 是拼接体的关键组成部分,对于mRNA前拼接至关重要.
  • 在拼接机制中,U5 snRNA的精确功能,特别是它的循环序列,仍然在很大程度上是未知的.
  • 之前的研究表明,它可能在拼接部位识别和拼接的催化步骤中发挥作用.

研究的目的:

  • 为了研究酵母U5 snRNA循环序列中特定突变的作用.
  • 为了阐明这些突变如何影响异常5'拼接部位的mRNA前裂变.
  • 了解U5 snRNA突变促进死胡同拉里亚特中间体的加工的机制.

主要方法:

  • 酵母U5 snRNA循环序列的局部定向突变发生.
  • 使用分子试验对前mRNA裂变产品和拼接中间体进行分析.
  • RNA-RNA基配对分析以确定U5 snRNA和前mRNA之间的相互作用.

主要成果:

  • 特定的U5 snRNA循环突变激活了突变前mRNA在异常的5'拼接部位的分裂.
  • 异常裂变的激活涉及U5 snRNA和上游前mRNA序列之间的基配对.
  • 突变促进了死胡同拉里亚特中间体的转化为mRNA,与U5snRNA基配对对应到第2个外显子.

更多相关视频

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

相关实验视频

Last Updated: May 13, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

结论:

  • U5 snRNA循环序列密切参与5'和3'拼接部位的转化反应.
  • 观察到的相互作用类似于II组自我拼接内核中的外子识别.
  • 这些发现支持了这样一个假设:U5 snRNA可能是从II组类内的功能域进化而来的.