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Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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相关实验视频

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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与肥胖相关的高血压的最佳治疗:高血压-肥胖-西布拉胺 (HOS) 研究.

Jürgen Scholze1, Elmar Grimm, Dana Herrmann

  • 1Department of Medicine, Outpatient Clinic, CCM, Charité-Universitätsmedizin Berlin, Luisenstrasse 11-13, 10117 Berlin, Germany. juergen.scholze@charite.de

Circulation
|April 4, 2007
PubMed
概括

对于肥胖的高血压患者来说,将西布胺与ACE抑制剂和通道阻断剂结合在一起,比β-阻断剂/利尿剂组合更能帮助减肥. 这种方法支持这种高危人群的代谢改善.

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相关实验视频

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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科学领域:

  • 心脏病学 心脏病学
  • 内分泌学 在内分泌学.
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 目前的高血压指南缺乏针对肥胖高血压患者的具体建议.
  • 肥胖和高血压经常并存,需要量身定制的治疗策略.
  • 研究药物相互作用对于优化这群人群的护理至关重要.

研究的目的:

  • 评估在肥胖高血压患者中西布拉明诱导的体重减轻的疗效.
  • 为了比较不同抗高血压疗法对sibutramine减肥作用的影响.
  • 确定最佳的抗高血压药物组合,以支持体重控制和代谢改善.

主要方法:

  • 展望性,16周,双盲,安慰剂控制,随机多中心研究.
  • 171名肥胖高血压患者接受了三种抗高血压疗法之一:费洛迪平/拉米普林,维拉帕米尔/特兰多拉普林,或美托普罗罗尔/甲.
  • 患者随机分配,接受西布胺 (15 mg) 或安慰剂.

主要成果:

  • 与安慰剂相比,Sibutramine显著降低了体重,BMI和腰围.
  • 用sibutramine减轻体重和减少内脏脂肪,而用metoprolol/hydrochlorothiazide则可以减轻这些效果.
  • 葡萄糖耐受性和过高甘油三血症的改善被美托普罗罗尔/酸胺废除.

结论:

  • 结合ACE抑制剂和通道阻断剂的抗高血压方案优于β-阻断剂/利尿剂组合,以支持西布拉明的减肥效果.
  • 这种组合疗法增强了肥胖高血压患者的代谢变化,这些患者接受了sibutramine治疗.
  • 这些发现有助于为肥胖高血压患者制定综合治疗策略.