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酵母菌RAD14和人体皮色素群A的DNA修复基因编码同源蛋白质

M Bankmann¹, L Prakash, S Prakash

¹Department of Biophysics, University of Rochester School of Medicine, New York 14642-8408.

|February 6, 1992

概括

Xeroderma pigmentosum (XP) 涉及到DNA修复缺陷的疾病. 酵母RAD14基因对于DNA切割至关重要,这是修复紫外线损伤的关键步骤,类似于人类XP基因.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

Xeroderma pigmentosum (XP) 是人类的一种自体相衰退性疾病.
XP的特点是极端的阳光敏感性和高皮肤癌发病率.
在紫外线损伤后,XP细胞在DNA切除修复的切口阶段表现出缺陷.

研究的目的:

描述酵母RAD14基因在DNA修复中的功能.
研究RAD14在核酸切除修复的切口阶段的作用.
为了将RAD14与人类XP基因进行比较.

主要方法:

酵母突变的遗传分析 (点和删除).
用DNA切口测试来评估修复能力.
对RAD14的蛋白质分析,包括序列和图案识别.

主要成果:

一个rad14点突变体表现出中度的紫外线敏感性和大量的DNA切口.
一种rad14删除突变体在DNA切割中证明了对RAD14的绝对要求.
RAD14编码了一种具有指图案的247氨基酸蛋白质,类似于人类的XPAC.

结论:

在酵母DNA修复的切口阶段,RAD14是必不可少的.
这些发现突显了酵母和人类DNA修复的遗传复杂性.
RAD14代表了人类XPAC基因的潜在功能同类物.

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