在携带反感性RNA转基因的小鼠中II型葡萄糖皮质体受体功能受损
M C Pepin1, F Pothier, N Barden
1Molecular Psychogenetics Laboratory, CHUL Research Center, Sainte Foy, Québec, Canada.
Nature
|February 20, 1992
概括
葡萄糖皮质体受体通过下丘脑-垂体-上腺轴调节压力反应. 这项研究使用了一种新的转基因小鼠模型来研究II型葡萄糖皮质体受体在这种反循环中的作用.
科学领域:
- 内分泌学 在内分泌学.
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 葡萄糖皮质类药物在下丘脑-垂体-上腺 (HPA) 轴上介导负反,调节上腺类固醇分泌.
- 已经确定了两种类型的皮质类固醇受体 (I型矿物质皮质类固醇和II型葡萄糖皮质类固醇),在皮质类固醇结合亲和力上有所不同.
- 二型葡萄糖皮质体受体参与终止应激反应,并且在皮质类固醇敏感度升高的情况下得到上调.
研究的目的:
- 研究II型葡萄糖皮质体受体在HPA轴的负反调节中的作用.
- 为了利用一种新型的转基因小鼠模型,其II型葡萄糖皮质体受体功能受损.
主要方法:
- 使用反感性RNA创建一个转基因小鼠模型,以部分淘汰II型葡萄糖皮质体受体基因表达.
- 在开发的转基因小鼠模型中分析HPA轴功能.
主要成果:
- 转基因小鼠模型显示皮质类固醇受体功能受损.
- 这种模型允许研究HPA轴上的葡萄皮质皮质体反机制.
结论:
- 第二种类型的皮质糖类受体在HPA轴的负反调节中发挥着重要作用.
- 开发的转基因小鼠是进一步研究葡萄糖皮质醇信号和应激反应的宝贵工具.
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