Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

11.4K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
11.4K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

5.5K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
5.5K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.0K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Adrenergic Receptors (Adrenoceptors): Classification01:27

Adrenergic Receptors (Adrenoceptors): Classification

4.9K
Adrenergic receptors, or adrenoceptors, respond to the autonomic neurotransmitter noradrenaline and other endogenous catecholamine agonists. They are classified into two main families, α and β, based on their pharmacological response and are further subdivided depending on their location, elicited response, and affinity to specific agonists or antagonists.
α-Adrenoceptors
α-Adrenoceptors are classified into two main subtypes: α1 and α2. The α1 adrenoceptors,...
4.9K
Adrenergic Receptors: ɑ Subtype01:31

Adrenergic Receptors: ɑ Subtype

2.7K
Adrenoceptors are classified into α and ꞵ classes based on their potencies to catecholamine agonists. α-adrenoceptors show the following order of catecholamine potency:
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase...
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatial statistics and point pattern analysis reveal lifespan trajectories of microglial density and clustering in the primate hippocampus.

Scientific reports·2026
Same author

Competing programs shape cortical sensorimotor-association axis development.

Nature·2026
Same author

Distinctive molecular mechanisms in higher cortical circuits confer vulnerability to mental disorders.

Biological psychiatry·2026
Same author

The MacBrain Resource Center (MBRC) rhesus macaque postnatal brain histology datasets: Enabling new discoveries through NHP tissue and digital data Repositories.

Journal of anatomy·2026
Same author

The MacBrain Resource Center (MBRC) rhesus macaque embryonic brain histology datasets.

Journal of anatomy·2026
Same author

Lamination of primary visual cortex in the macaque: Layer 5 subdivisions.

Journal of anatomy·2026

相关实验视频

Updated: May 3, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

14.1K

阿尔法2A上腺受体通过抑制前额叶皮层中的cAMP-HCN通道信号来加强工作记忆网络.

Min Wang1, Brian P Ramos, Constantinos D Paspalas

  • 1Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510 USA.

Cell
|April 24, 2007
PubMed
概括

刺激alpha2A adrenoceptors通过抑制cAMP,关闭HCN通道并增强前额叶皮层网络连接性来加强空间工作记忆 (WM). 这种机制提高了WM的性能.

更多相关视频

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.7K
Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.8K

相关实验视频

Last Updated: May 3, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

14.1K
Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.7K
Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.8K

科学领域:

  • 神经科学是一个神经科学.
  • 认知神经科学 认知神经科学
  • 分子神经科学 分子神经科学

背景情况:

  • 空间工作记忆 (WM) 依赖于前额叶皮层 (PFC) 神经网络.
  • 阿尔法2A上腺受体 (alpha2A-ARs) 在WM维护中起着至关重要的作用.
  • 在PFC中空间调节的神经元活动在延迟期间是WM的基础.

研究的目的:

  • 阐明α2A-AR刺激增强空间WM的分子机制.
  • 调查cAMP和高极化激活循环核酸门 (HCN) 通道在WM调节中的作用.
  • 为了确定调制HCN通道对PFC网络功能和WM性能的影响.

主要方法:

  • 在PFC中对HCN通道和alpha2A-AR进行超结构同位化分析.
  • 电生理学记录以评估神经元触发和网络活动.
  • 行为实验涉及PFC中HCN1通道的药理阻断或遗传淘汰.

主要成果:

  • 阿尔法2A-AR刺激抑制了cAMP,导致HCN通道关闭,并加强了PFC网络连接.
  • HCN通道阻塞或cAMP抑制增强了空间调节,延迟相关的PFC神经元的发射.
  • 在PFC中阻断或淘汰HCN1通道显著改善了WM性能.

结论:

  • 阿尔法2A-ARs通过调节PFC神经元中的cAMP水平和HCN通道活性来加强空间WM.
  • HCN通道是PFC网络功能的关键调节者,对空间WM至关重要.
  • 针对alpha2A-AR/cAMP/HCN通路提供了增强WM的潜在策略.