一种III型效应剂ADP-ribosylatesRNA结合蛋白,并抑制植物免疫力
Zheng Qing Fu1, Ming Guo, Byeong-ryool Jeong
1Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68588-0660, USA.
Nature
|April 24, 2007
概括
细菌病原体Pseudomonas syringae通过修改RNA结合蛋白来使用HopU1效应体来抑制植物免疫力. 这种ADP-ribosylation影响RNA代谢和植物防御基因表达.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- Pseudomonas syringae通过通过III型分泌系统注入效应蛋白来引起植物疾病.
- 大多数P.注射器效应器的功能及其分子点在很大程度上是未知的.
研究的目的:
- 为了阐明P.注射器III型效应器HopU1.1.的酶活性和功能.
- 确定HopU1的宿主目标,并了解其在植物免疫抑制中的作用.
主要方法:
- 生物化学测试以确定HopU1作为单-ADP-ribosyltransferase (ADP-RT) 的酶活性.
- 在Arabidopsis thaliana提取物中使用生物化学方法识别HopU1基质.
- 对P. syringae感染的易感性进行Arabidopsis thaliana淘汰线 (例如,grp7) 的分析.
主要成果:
- HopU1被确定为一种抑制植物先天免疫力的单-ADP-ribosyltransferase (ADP-RT).
- HopU1针对含有RNA识别基因 (RRMs) 的RNA结合蛋白,包括GRP7 (AtGRP7).
- 缺乏GRP7的阿拉比多普西斯植物对P. syringae的敏感性增加,并且HopU1介导的GRP7的ADP-ribosylation干扰了RNA结合.
结论:
- HopU1使用宿主RNA结合蛋白的ADP-核糖化来破坏RNA代谢并抑制植物免疫力.
- 这种机制突出显示了一种涉及植物防御转录组的新型致病策略.
- 了解HopU1的功能,可以了解细菌的毒性和植物的免疫反应.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Defenses Against Pathogens and Herbivores
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...


