在流体中称重生物分子,单细胞和单纳米粒子
Thomas P Burg1, Michel Godin, Scott M Knudsen
1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nature
|April 27, 2007
概括
研究人员开发了一种新的微通道共振器,用于衡量液体中的微小粒子. 这一突破实现了子图分辨率,克服了纳米技术和生命科学应用在流体环境中的先前限制.
科学领域:
- 纳米技术纳米技术
- 生物物理学的生物物理.
- 分析化学 分析化学
背景情况:
- 纳米机械共振器提供高质量灵敏度,但流体粘度限制了它们的性能.
- 以前的方法在溶液中的质量测量方面遇到了困难,原因是共振器质量因子和质量降低.
研究的目的:
- 开发一种能够在液体中进行高分辨率质量测量的纳米机械共振器.
- 为了克服流体环境中粘度所带来的限制.
主要方法:
- 使用悬浮微通道共振器,其设计是空洞的,放置在真空中.
- 通过将充满流体的通道与周围的真空隔离,最大限度地降低粘性缓.
主要成果:
- 在水中实现了单个纳米粒子,细菌细胞和蛋白质子单层的子乳图质量分辨率.
- 与共振器的内在减噪相比,显示的粘性损失可以忽略不计.
- 与石英晶体微平衡相比,质量分辨率提高了六个数量级.
结论:
- 悬浮微通道共振器能够在液体中实现前所未有的质量灵敏度.
- 这项技术为基于质量的流细胞计和病原体检测等应用打开了大门.
相关概念视频
05:12Murine Nasal Lavage Fluid Collection without Blood Contamination
1.7K
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
1.7K
03:40Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
1.1K
OSTSS leads to pooled inflammatory mediators in the tear film, causing symptoms like epiphora and discomfort. Here, we present a case where therapeutic nasolacrimal lavage every 2 months resolved epiphora and itchiness, suggesting its efficacy as a therapeutic intervention for OSTSS. Additionally, we report subjective improvements in symptoms in 3 additional...
1.1K
04:35Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
880
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
880
08:47Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
2.9K
A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental...
2.9K
06:49Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
12.0K
Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment...
12.0K
07:15Intranasal Administration of CNS Therapeutics to Awake Mice
61.4K
A method to intranasally administer drugs to awake mice for the purpose of targeting the brain is described. This method allows for repeat dosing over long periods using intranasal administration of drug without anesthesia, and nose-to-brain delivery with minimal systemic...
61.4K