控制替代RNA剪接和基因表达的控制由真核细胞的核糖核酸切换器
Ming T Cheah1, Andreas Wachter, Narasimhan Sudarsan
1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Nature
|May 1, 2007
概括
细胞使用称为 рибо开关的代谢物结合RNA来控制基因表达. 在真菌中,氨酸酸盐 (TPP) рибо开关调节信使RNA拼接,影响TPP代谢.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 欧核生物基因调节 欧核生物基因调节
背景情况:
- 细菌利用 рибо开关来感知代谢物和调节基因表达,主要是通过转录终止或翻译启动.
- 氨酸酸盐 (TPP) рибо开关,响应维生素B1衍生物,在细菌中广泛存在,并预测可以控制真核生物中的RNA处理.
- 据假设,真核TPP核糖突变器会影响信使RNA (mRNA) 的拼接或处理.
研究的目的:
- 为了研究TPP带状交换机在线状真菌Neurospora crassa中的功能.
- 为了确定TPP核糖开关如何调节基因表达在真核生物的mRNA拼接水平.
- 阐明在TPP新陈代谢中通过 рибо开关介导的替代拼接控制的机制.
主要方法:
- 在Neurospora crassa.中对三种TPP核糖开关进行分析.
- 对基因表达调节的实验性检查.
- 详细的机理学研究 riboswitch介导的基配对变化和替代拼接为NMT1前体mRNAs.
主要成果:
- 发现一个TPP核突变器激活了基因表达,而两个则抑制了它,所有这些都是通过控制mRNA拼接来实现的.
- 对前体NMT1mRNAs阐明了一种特定的机制,证明了 рибо开关对替代拼接的控制.
- 这些发现突显了TPP核糖开关在通过拼接调制调节参与TPP代谢的基因调节中的作用.
结论:
- 细胞使用代谢物感应RNA (riboswitches) 来调节RNA剪接.
- 在真菌中,TPP的核糖开关通过调节替代的mRNA拼接来控制基因表达.
- 这种机制对于调节真核生物体中关键的生物化学过程至关重要,例如TPP代谢.
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