相关实验视频
Updated: Jul 11, 2026
08:40
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
微RNA-223表达的进化保守机制由微RNA基因分析揭示出来
Taro Fukao1, Yoko Fukuda, Kotaro Kiga
1Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. tarofukao@2002.jukuin.keio.ac.jp <tarofukao@2002.jukuin.keio.ac.jp>
Cell
|May 8, 2007
概括
这项研究揭示了髓状细胞中微RNA-223 (miR-223) 的新型调节机制. miR-223基因由髓状转录因子控制,与以前已知的途径不同.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- 微RNAs (miRNAs) 显示保存的序列和特定的表达模式.
- 组织特定的miRNA表达通常由miRNA基因附近的cis作用元素调节.
研究的目的:
- 为了确定控制miRNA表达的调节元素.
- 为了阐明微RNA-223 (miR-223) 在颗粒形成过程中的独特调节机制.
主要方法:
- 生物信息分析以确定miRNA转录起点附近的保存基因组元素.
- 研究了髓质转录因子 (PU.1和C/EBPs) 在调节miR-223中的作用.
主要成果:
- 确定了近于miR-223的保守的cis-regulatory元素.
- 发现miR-223的表达可能是由髓质转录因子PU.1和C/EBPs驱动的.
- 为miR-223提出了一种与之前描述的途径不同的新型调节机制.
结论:
- 该miR-223基因作为"骨髓基因"的功能,由特定的转录因子调节.
- 一个保存的cis-regulatory元素很可能控制着这种跨物种的骨髓特异性调节.
- 两种不同的机制可能控制miR-223,在骨髓细胞功能和分化中发挥关键作用.
相关概念视频
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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...