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Updated: Jul 15, 2026

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

在溶液和气相中的Trp-cage蛋白质构成的光探针

Anthony T Iavarone1, Alexandra Patriksson, David van der Spoel

  • 1Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, Massachusetts 02142, USA.

Journal of the American Chemical Society
|May 10, 2007
PubMed
概括

通过比较气相和溶液蛋白展开,可以发现关键的稳定性驱动因素. 键主导了气相稳定性,而盐桥在Trp小蛋白的溶液中至关重要.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...