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Updated: Jul 15, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
12:31

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

模板导向的寡核酸链绑定,利用点击化学的共价内分子DNA循环化和链接

Ravindra Kumar1, Afaf El-Sagheer, John Tumpane

  • 1School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.

Journal of the American Chemical Society
|May 10, 2007
PubMed
概括

铜催化点击化学可以使DNA结合,创造新的脊椎. 这种方法还促进了无模板DNA循环化和链合成.

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Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes05:33

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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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