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相关概念视频

Fischer Projections02:18

Fischer Projections

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Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines.
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Chromatin Immunoprecipitation- ChIP02:36

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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相关实验视频

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Preparation and Characterization of SDF-1&#945;-Chitosan-Dextran Sulfate Nanoparticles
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通过Sdp1的氧转化介导基质识别定义了一组新型的氨酸酸酶.

G C Fox1, M Shafiq, D C Briggs

  • 1Structural Biology Laboratory, The London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.

Nature
|May 15, 2007
PubMed
概括

这项研究揭示了酵母MAPK酸酶Sdp1如何在氧化应激下独特地增强其活性. 这一发现为调节应激反应信号通路提供了一种新的机制.

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科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 分子生物学分子生物学

背景情况:

  • 反应性氧物种 (ROS) 激活了应激反应的基因激活蛋白激酶 (MAPK) 途径.
  • 关闭MAPK通路需要基于氨酸的酸酶,但氧化应激通常会使这些酶失活.
  • 这造成了一个悖论,氧化应激可能导致不受控制的MAPK激活.

研究的目的:

  • 研究应激诱导的MAPK酸酶Sdp1在氧化条件下发挥作用的机制.
  • 了解Sdp1如何克服氧化应激引起的酸酶的悖论性失活.
  • 将Sdp1描述为压力信号的新型调节器.

主要方法:

  • 对Sdp1.1的结构和生化分析.
  • 在不同的氧化条件下对酶活性进行测定.
  • 使用氨酸酸化MAPK进行基质识别研究.
  • 与Sdp1同类Msg5.5进行比较分析.

主要成果:

  • 在氧化条件下,sdp1表现出增强的催化活性,与典型的基于氨酸的酸酶不同.
  • 在Sdp1的基质识别和活性中,一个分子内部的二硫化物桥梁和一个胺残留物至关重要.
  • Sdp1可以选择性地结合和去酸化氨酸酸化的MAPK基质.
  • Sdp1和Msg5代表了在酵母和真菌中发现的新类酸酶.

结论:

  • 在氧化应激过程中,Sdp1为酸酶调节的悖论提供了一个独特的解决方案.
  • 该酶通过其独特的结构特征将氧化应激与特定基质识别相结合.
  • Sdp1和Msg5突出了一个新的酸酶组,在真菌应激反应中具有潜在的作用.