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High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
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通过循环偏光探测超分子性堆中的激发移位:实验和建模

Frank C Spano1, Stefan C J Meskers, Emanuelle Hennebicq

  • 1Department of Chemistry, Temple University, Beury Hall 201, Philadelphia, Pennsylvania 19122, USA. spano@temple.edu

Journal of the American Chemical Society
|May 15, 2007
PubMed
概括

研究人员研究了性寡烯烯 (MOPV) 聚合物的光刺激,揭示了电子振动合和乱如何定位刺激子. 这项工作模拟了光中波长依赖的圆极化,为激发状态动态提供了洞察力.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...