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通过在位RAFT聚合物化的精确定义的蛋白质聚合物结合物
Cyrille Boyer1, Volga Bulmus, Jingquan Liu
1Centre for Advanced Macromolecular Design (CAMD), School of Chemical Sciences and Engineering, The University of New South Wales, Sydney NSW 2052 Australia.
Journal of the American Chemical Society
|May 16, 2007
概括
本研究引入了一步方法,使用可逆添加碎片化链转移聚合 (RAFT) 制造明确的蛋白质聚合物结合物. 这种高效的技术避免了复杂的净化,为生物技术应用产生具有保留蛋白活性的同质结合物.
科学领域:
- 生物结合化学 生物结合化学
- 聚合物科学 聚合物科学
- 生物技术是生物技术.
背景情况:
- 在生物技术,生物医学和纳米技术的先进应用中,开发均的蛋白质聚合物合物至关重要.
- 现有的方法往往涉及复杂和耗时的净化步骤,限制了它们的效率.
研究的目的:
- 报告使用可逆添加-碎片化链转移聚合 (RAFT) 来有效地在现场合成明确的蛋白质聚合物联合体的一步.
- 为了证明在没有主要的聚合后净化的情况下生成同质合物.
主要方法:
- 通过其Cys-34醇组将水溶性RAFT剂与牛血清白蛋白 (BSA) 相结合.
- 在现场控制的聚合N-异烯胺 (NIPAAm) 和乙烯酸 (HEA) 使用BSA-macroRAFT剂.
- 使用紫外线可见光谱学,MALDI-TOF,1H NMR,SEC和PAGE进行了表征.
主要成果:
- 成功合成高效率的BSA-聚合物联合体 (大约. 1). 1). 一个 1). 一个 1).
- 通过线性分子量演变和没有自由聚合物证明受控的聚合.
- 在结合后不影响BSA的结构完整性和酶活性.
- BSA-poly ((NIPAAm) 结合物表现出混合体的温度依赖行为.
结论:
- 在现场的RAFT聚合提供了一条有效的途径,以获得均的蛋白质-聚合物结合物.
- 该方法保留了蛋白质的结构和功能,使其适合各种应用.
- 由此产生的合物显示可调节的特性,例如温度依赖的相位分离.
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