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相关概念视频

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Downsampling01:20

Downsampling

When considering a sampled sequence with zero values between sampling instants, one can replace it by taking every N-th value of the sequence. At these integer multiples of N, the original and sampled sequences coincide. This process, known as decimation, involves extracting every N-th sample from a sequence, thereby creating a more efficient sequence.
The Fourier transform of the decimated sequence reveals a combination of scaled and shifted versions of the original spectrum. This...

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相关实验视频

Updated: Jul 10, 2026

Presynaptically Silent Synapses Studied with Light Microscopy
11:02

Presynaptically Silent Synapses Studied with Light Microscopy

Published on: January 4, 2010

在沉默中沉默.

Elizabeth H Bayne1, Sharon A White, Robin C Allshire

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, Kings Buildings, Michael Swann Building, Mayfield Road, Edinburgh, EH9 3JR, UK.

Cell
|May 22, 2007
PubMed
概括

RNA干扰机器直接准裂变酵母中的转基因转录,将沉默与TRAMP类复合物联系起来. 这表明RNAi独立的转录降解有助于异性染色体基因沉默.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 在裂变酵母中,由RNA干扰 (RNAi) 介导的 heterochromatin 形成使转基因在中间体处沉默.
  • 将RNAi与异染色体和转基因沉默联系在一起的精确机制正在研究中.

研究的目的:

  • 研究转基因沉默中RNA干扰机制的直接目标.
  • 探索蛋白质复合体在依赖RNAi的异染色素形成和基因沉默中的作用.

主要方法:

  • 在裂变酵母中分析RNA干扰路径.
  • 通过RNAi机器对转基因转录的向的研究.
  • 参与转录降解的蛋白质复合体的表征.

主要成果:

  • RNAi机制直接针对转基因转录物进行降解.
  • 一个类似于芽酵母中TRAMP复合体的蛋白质复合体与转基因沉默有关.
  • 这种复合物促进了通过外体的转录降解.

结论:

  • RNA干扰机器直接与转基因转录进行交互.

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相关实验视频

Last Updated: Jul 10, 2026

Presynaptically Silent Synapses Studied with Light Microscopy
11:02

Presynaptically Silent Synapses Studied with Light Microscopy

Published on: January 4, 2010

Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats
11:00

Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats

Published on: August 8, 2011

Systematic Hearing Performance Evaluation Process for Adolescents with Cochlear Implantation at Early Ages
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Published on: March 24, 2023

  • 转基因沉默涉及一种类似TRAMP的复杂和外基因介导的降解.
  • 独立于RNAi的转录降解途径可能有助于异性染色素介导的基因沉默.