在人类基因组中,高分辨率的基因组甲基化概况
Artem Barski1, Suresh Cuddapah, Kairong Cui
1Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
Cell
|May 22, 2007
概括
这项研究绘制了全人类基因组中的基因组修饰图,揭示了与基因激活和抑制相关的独特模式. 这些发现为染色体组织和基因组功能提供了新的见解.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 基因组蛋白修饰在调节基因表达中起着至关重要的作用.
- 了解这些修饰的全基因组分布对于破译基因调节至关重要.
- 之前的研究已经提供了对基因素修饰模式的碎片化观点.
研究的目的:
- 为了生成高分辨率,全基因组地图的20个基因组甲基化,H2A.Z,RNA聚合酶II和CTCF在人类基因组.
- 识别基因组的关键元素,如促进剂,增强剂和绝缘剂的典型的组织基因组甲基化模式.
- 为了与染色体结构和与癌症相关的断点相关联的基因组修饰模式.
主要方法:
- 使用Solexa 1G测序技术进行高分辨率映射.
- 对20种基因素氨酸和氨酸甲基化进行了全基因组分布分析.
- 综合数据与RNA聚合酶II,H2A.Z和CTCF结合配置文件.
主要成果:
- 鉴定出与基因激活 (例如,H3K27me1,H3K9me1) 和抑制 (例如,H3K27me3,H3K9me3) 相关的独特的组织基因甲基化模式.
- 证明H2A.Z与功能性监管元素有关.
- 显示的CTCF标记了基因素甲基化域的边界,并发现了染色体带和基因素修饰之间的相关性.
- 观察到T细胞癌症中的染色体断点经常发生在H3K4甲基化区域.
结论:
- 基因组甲基化模式与基因激活和抑制状态密切相关.
- 基质子修饰和像H2A.Z和CTCF这样的蛋白质是染色质组织中的关键参与者.
- 这种高分辨率的映射提供了对基因组调节和疾病中的基因组甲基化功能作用的关键见解.
更多相关视频
11:02Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
Published on: May 17, 2016
30.8K
24:02The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
Published on: April 12, 2014
18.9K
相关概念视频
02:32Histone Modification
17.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
17.2K
02:32Histone Modification
4.9K
No description available
4.9K
02:38Heterochromatin
19.0K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
19.0K
02:25Spreading of Chromatin Modifications
10.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
10.0K