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在哺乳动物体外系统中实时光检测ERAD基质逆转移

Judit Wahlman1, George N DeMartino, William R Skach

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA.

Cell
|June 2, 2007
PubMed
概括

在内质网膜 (ER) 中错误折叠的分泌蛋白经历了与ER相关的降解 (ERAD). 研究人员发现derlin-1,而不是Sec61alpha,对于ERAD期间的pro-alpha因子的逆转移至关重要.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...