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控制DNA甲基化和异色沉默的控制通过素H2B二氧化化
Vaniyambadi V Sridhar1, Avnish Kapoor, Kangling Zhang
1Center for Plant Cell Biology and Department of Botany and Plant Sciences.
Nature
|June 8, 2007
概括
在SUP32/UBP26酶的突变破坏了基因组修饰,影响DNA甲基化和基因沉默在Arabidopsis. 这项研究揭示了基因组H2B二维基因化与表观遗传基因调节之间的联系.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物科学 植物科学
背景情况:
- 表观遗传调节涉及可逆的DNA甲基化和基因素修饰.
- 短干扰RNAs (siRNAs) 通过DNA甲基化和异色色素基因标记来调解序列特定的基因沉默.
- 已知Histone H2B monoubiquitination可以调节动物和酵母中的Histone H3甲基化,但其与DNA甲基化的联系尚未被探索.
研究的目的:
- 为了研究在Arabidopsis. histone中基因化和DNA甲基化之间的关系.
- 阐明二维基因化酶SUP32/UBP26在表观遗传基因沉默中的作用.
主要方法:
- 对缺乏SUP32/UBP26功能的阿拉比多普西斯突变物进行分析.
- 染色体免疫沉测定用于评估基因素修饰和无处不在.
- 对DNA甲基化模式的量化.
主要成果:
- 在SUP32/UBP26中发生的突变导致H3氨酸9二甲基化减少,并抑制siRNA导向的DNA甲基化.
- 转基因和转子体的异色沉默在sup32突变体中被释放出来.
- 阿拉比多普西斯菌组合体H2B在lysine143处单基化; sup32突变体显示在lysine4三甲基化时,ubiquitinated H2B和H3的基化增加.
- SUP32/UBP26二基化H2B,而H2B的二基化与沉声的释放有关.
结论:
- 通过SUP32/UBP26进行H2B二维基因化对于异色色素组合素H3甲基化和DNA甲基化至关重要.
- 这项研究揭示了一种新的机制,该机制将素H2B二维基因化与植物表观遗传沉默的建立和维护联系起来.
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