基因表达中的噪音决定了Bacillus subtilis中的细胞命运
Hédia Maamar1, Arjun Raj, David Dubnau
1Public Health Research Institute Center, New Jersey Medical School, 225 Warren Street, Newark, NJ 07103, USA.
概括
细胞对细胞的基因表达变异,或噪声,驱动转换到Bacillus subtilis的能力. 减少这种噪声会减少有能力的细胞,显示噪声.
科学领域:
- 微生物学 微生物学
- 系统生物学 系统生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞过程在同源种群中表现出随机基因表达变异 (噪音),可能影响状态过渡.
- 噪声在自然生物系统中的作用,特别是调节基因表达动态,仍然在很大程度上未被探索.
- 在 Bacillus subtilis 中,ComK (能力调节蛋白) 中的噪音被假设为驱动向DNA吸收能力状态的过渡.
研究的目的:
- 调查噪声对comK表达对细胞过渡到Bacillus subtilis能力的影响.
- 为了确定噪声水平的实验性操纵是否会影响主管单元的频率.
- 阐明管理对能力的随机过渡的时间调节机制.
主要方法:
- 在bacillus subtilis.comK基因表达中量化和操纵噪声.
- 对comK表达噪声的实验性降低.
- 分析comK转录和能力转换的时间动态.
主要成果:
- 在comK表达中的噪声积极选择向能力过渡的细胞.
- 实验性减少comK表达噪声显著降低了合格细胞的比例.
- 随机过渡到能力是暂时受到限制的减少comK转录.
结论:
- 基因表达中的噪音在自然系统中调节静态细胞状态转换方面发挥着至关重要的作用.
- 噪声的特征受到进化压力,优化像竞争力这样的细胞功能.
- 了解噪声动态,可以深入了解基因表达和细胞分化的调节.
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