来自纳米升水滴的功能生物网络
Matthew A Holden1, David Needham, Hagan Bayley
1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, OX1 3TA Oxford, UK. matthew.holden@chem.ox.ac.uk
Journal of the American Chemical Society
|June 19, 2007
概括
研究人员使用滴滴接口二层 (DIB) 创建了强大的生物网络. 这些网络作为合的原细胞起作用,为超敏感双层记录提供稳定的平台,使快速选应用成为可能.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 合成生物学 合成生物学
背景情况:
- 脂质双层对细胞功能和仿生系统至关重要.
- 创建稳定,功能性的人工细胞网络仍然是一个重大挑战.
研究的目的:
- 开发一种方法,使用脂质双层构建稳健的,相互连接的水滴网络.
- 调查这些滴滴网络作为生物网络和记录设备的功能能力.
主要方法:
- 通过将水滴浸入油脂混合物中,形成液滴接口二层 (DIB).
- 将蛋白质通道和毛孔纳入DIB用于离子电流测量.
- 组装线性和分支滴滴网络几何形状.
- 利用离子梯度或背后的hodopsin为网络提供动力.
主要成果:
- 证明了稳定和强大的滴状网络的形成,在接口处具有脂质二层.
- 展示了结合功能蛋白通道和测量跨DIBs的离子电流的能力.
- 在不损害结构完整性的情况下,成功切除并替换网络中的单个滴.
- 开发了使用内部生物电池为这些生物网络提供动力的方法.
结论:
- 滴滴接口二层为创建功能性生物网络提供了一个稳定和多功能平台.
- 这些网络可以作为合的原细胞和超稳定的双层记录的先进平台.
- 该技术有望用于快速选和仿生系统中的应用.
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