Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
Asexual Reproduction02:38

Asexual Reproduction

Asexual reproduction allows plants to reproduce without growing flowers, attracting pollinators, or dispersing seeds. Offspring are genetically identical to the parent and produced without the fusion of male and female gametes.
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Genome Copying Errors02:46

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Author Correction: Autophagic cell death restricts chromosomal instability during replicative crisis.

Nature·2026
Same author

Centromeric footprints preserve telomere integrity in ALT cancers.

Nature·2026
Same author

Unbalanced chromatin binding of Polycomb complexes drives neurodevelopmental disorders.

Molecular cell·2026
Same author

Bypass of blocking lesions by RNAPII reveals a novel stress induced by DNA damage.

Genes & development·2026
Same author

Telomere Crisis Shapes Cancer Evolution.

Cold Spring Harbor perspectives in biology·2025
Same author

Telomeres at the nexus of aging, tumor suppression, and inflammation: toward an understanding beyond senescence.

Genes & development·2025

相关实验视频

Updated: Jul 12, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

端粒的复制和保护 端粒的复制和保护

Ramiro E Verdun1, Jan Karlseder

  • 1The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA.

Nature
|June 22, 2007
PubMed
概括

端粒保护染色体末端免受DNA损伤和缩短. 这些结构与DNA复制和修复系统相互作用,对基因组稳定性至关重要.

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 线性基因组需要称为端粒的特殊结构来保护染色体末端.
  • 端粒由重复的DNA序列和相关蛋白质组成,防止不必要的DNA损伤反应.
  • 这些结构也起到缓冲作用,防止DNA复制过程中发生的缩短.

研究的目的:

  • 讨论端粒在维护基因组完整性方面的重要作用.
  • 探索端粒与DNA复制机制之间的复杂相互作用.
  • 阐明端粒和DNA损伤修复通路之间的合作.

主要方法:

  • 审查现有的文献和模型生物研究.
  • 对调节端粒维持的分子机制的分析.
  • 比较基因组学和功能研究.

主要成果:

  • 端粒成功地封闭了染色体末端,防止它们在DNA断裂时被识别.
  • 端粒可以抵消染色体在每一轮DNA复制过程中逐渐缩短的作用.
  • 端粒结合蛋白和复制/修复因子之间存在复杂的相互作用.

结论:

更多相关视频

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
04:09

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

相关实验视频

Last Updated: Jul 12, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
04:09

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

  • 端粒维护对于线性基因组的稳定性至关重要.
  • 了解端粒复制-修复相互作用是理解基因组稳定性的关键.
  • 功能障碍的端粒与衰老和癌症等疾病有关.