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相关概念视频

RNA Structure01:23

RNA Structure

69.1K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
69.1K
RNA Stability01:53

RNA Stability

31.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
31.7K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

10.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
10.3K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
RNA Structure01:19

RNA Structure

6.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K

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相关实验视频

Updated: May 6, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

7.4K

细菌RNA聚合酶中基质负荷的结构基础

Dmitry G Vassylyev1, Marina N Vassylyeva, Jinwei Zhang

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Schools of Medicine and Dentistry, 402B Kaul Genetics Building, 720 20th Street South, Birmingham, Alabama 35294, USA. dmitry@uab.edu

Nature
|June 22, 2007
PubMed
概括

在RNA聚合酶中的触发环重新折叠机制对于基质加载至关重要. 结构研究揭示了不同的预插入和插入状态,为抗生素开发提供了目标.

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 分子生物学分子生物学

背景情况:

  • 了解RNA聚合酶基质负载是转录的关键.
  • 精确的机制仍然是辩论的主题.

研究的目的:

  • 为了阐明多子单元RNA聚合酶中基质加载的机制.
  • 为了确定基质结合和抑制的结构基础.

主要方法:

  • 在3.0A分辨率的X射线晶体学.
  • 生物化学测试. 生物化学测试.

主要成果:

  • 确定了Thermus thermophilus延长复合体 (EC) 与AMPcPP和AMPcPP/链球素的结构.
  • AMPcPP通过触发环 (TL) 重复折叠在一个活跃的"插入"部位上结合.
  • 斯特雷普托利迪丁通过取代TL.稳定了不活跃的"预插入"状态.

结论:

  • 在RNA聚合酶催化过程中,TL重折是必不可少的.
  • 两步预插入/插入机制用于基板加载可能是通用的.
  • 预插入状态是新型抗生素的潜在目标.

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