证据表明,在气相中存在状结构
Michal Sharon1, Leopold L Ilag, Carol V Robinson
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Journal of the American Chemical Society
|June 26, 2007
概括
电子喷雾质谱学揭示了表面活性物如何在微粒中定位和逆向微粒. 这种方法证实了表面活性剂与囊括蛋白质 (如肌球蛋白) 的相互作用在分析过程中保持不变.
科学领域:
- 分析化学 分析化学
- 生物物理化学 生物物理化学
- 超分子化学 超分子化学
背景情况:
- 微粒和逆微粒是具有明显表面活性剂排列的关键超分子结构.
- 了解这些组件中的表面活性剂行为和相互作用对于各种应用至关重要.
- 电子喷射质谱 (ES-MS) 为分析溶液中的非共价复合物提供了一个强大的工具.
研究的目的:
- 使用ES-MS比较微粒,反向微粒和肌球蛋白封装反向微粒的行为.
- 为了研究表面活性剂在电喷射过程中保持面向和离子交换.
- 为了确定与封装的肌球蛋白相关的表面活性分子的固体几何学.
主要方法:
- 使用电子喷雾质谱法 (ES-MS) 来分析菌和逆菌制剂.
- 采用双重质谱法 (MS/MS) 来探测离子解离和头组交换.
- 通过调整水和有机相组合物,使用 cetyltrimethylammonium bromide (CTAB) 作为表面活性剂来控制小的形成.
主要成果:
- 正常的菌体显示出大量的酸离子交换 (>90%),而反向菌体显示出最小的交换,表明头组保护.
- 双重质谱揭示了两种小细胞类型中数百个CTAB分子的多分散组合.
- 囊括髓蛋白的反向微粒含有大约270个CTAB分子,每一个全体髓蛋白分子.
结论:
- 表面活性剂的溶液相位方向在电喷射电离过程中保持.
- 表面活性剂与封装蛋白质的相互作用在整个ES-MS过程中保持不变.
- ES-MS是一种合适的技术,用于表征自组装表面活性剂系统和蛋白质-表面活性剂复合物的结构和固态度.
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