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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Implementation of a Reference Interferometer for Nanodetection
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可调节的纳米线非线性光学探头.

Yuri Nakayama1, Peter J Pauzauskie, Aleksandra Radenovic

  • 1Department of Chemistry, University of California, USA.

Nature
|June 29, 2007
PubMed
概括

研究人员使用酸纳米线开发了一个可调节的,无电极的连贯可见光源. 这一突破使得亚波长显微镜能够与生理条件相兼容,进步光学科学.

科学领域:

  • 亚波长光学是低波长的光学.
  • 纳米光子学 纳米光子学
  • 光学显微镜是一种光学显微镜.

背景情况:

  • 开发在室温和生理条件下稳定的可调整的连贯激光源对亚波长光学来说是一个挑战.
  • 现有的先进成像技术可以实现纳米分辨率,但生物应用仍然存在局限性.

研究的目的:

  • 为了创建一个连续调节的,无电极的连贯可见光源.
  • 使用这种光源实现一种新的亚波长显微镜技术.

主要方法:

  • 作为核心组件使用单个酸 (KNbO3) 纳米线.
  • 杆式第二波生成和总和/差异频率生成用于色调调性.
  • 采用红外激光用于光学捕捉和扫描样本上的纳米线.

主要成果:

  • 从KNbO3纳米线证明了一个无电极,可连续调节的连贯可见光源.
  • 通过频率转换实现了各种颜色的局部合成.
  • 成功实施了一种新的亚波长显微镜技术.

结论:

  • 酸纳米线为可调节,生理兼容的连贯光源提供了一个有前途的平台.

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  • 开发的纳米光源使新的亚波长显微镜形式成为可能.
  • 潜在的应用范围包括物理,化学,材料科学和生物学.