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从哺乳动物胚胎中衍生出多能表皮质干细胞
I Gabrielle M Brons1, Lucy E Smithers, Matthew W B Trotter
1Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke's Hospital, University of Cambridge, Cambridge CB2 0XY, UK.
Nature
|June 29, 2007
概括
研究人员使用化学定义的介质从小鼠和老鼠胚胎中获得了新的多能干细胞. 这一发现突显了活性蛋白/节点信号传导在维持物种间多能性的保留作用.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 比较生物学的比较生物学
背景情况:
- 胚胎干细胞 (ES) 的衍生在老鼠以外的哺乳动物中具有挑战性.
- 尽管人类和小鼠的ES细胞具有相似的多能性,但它们在维护过程中使用不同的信号通路.
- 鼠标ES细胞需要LIF和BMP;人类ES细胞依赖于Activin/Nodal和FGF.
研究的目的:
- 通过使用化学定义的介质,从植入后的哺乳动物胚胎中获得和维持多能干细胞.
- 为了研究激素/节点信号传导在不同物种多能性中的作用.
- 建立一个系统来比较干细胞生物学中的特定物种差异.
主要方法:
- 从植入后小鼠和老鼠胚胎的表皮质中提取多能干细胞.
- 使用含有activin的化学定义培养基.
- 评估了衍生干细胞的长期维持和多能性.
主要成果:
- 成功地从老鼠和老鼠胚胎的晚期表皮质中获得多能干细胞.
- 化学定义,含有活性素的介质支持多能性的长期维持.
- 证明了Activin/Nodal信号传导在干细胞衍生和维护中的进化性保存作用.
结论:
- 活性蛋白/节点信号传递对于在新型表皮质干细胞中获得和维持多能性至关重要.
- 表皮质干细胞提供了一个模型来探索ES细胞中特定物种的区别与时间起源差异.
- 这项工作扩大了跨哺乳动物物种的干细胞研究工具包.
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