在细菌中进行基因组移植:将一种物种转换为另一种物种
Carole Lartigue1, John I Glass, Nina Alperovich
1J. Craig Venter Institute, Rockville, MD 20850, USA.
Science (New York, N.Y.)
|June 30, 2007
概括
科学家将一种细菌基因组移植到另一种物种中,创造了一个具有合成基因组的细胞. 合成生物学中的这一突破为创造人工生命形式铺平了道路.
科学领域:
- 合成生物学 合成生物学
- 微生物基因组学 微生物基因组学
- 细胞重新编程的细胞重编程.
背景情况:
- 细菌基因组移植是一个复杂的过程.
- 以前的方法在实现完整的基因组替换方面存在局限性.
研究的目的:
- 为了证明细菌细胞的基因组与捐赠者的基因组的完全替换.
- 建立一种创建具有合成基因组的细胞的方法.
主要方法:
- 将完整的Mycoplasma mycoides大殖民地 (LC) 基因组DNA移植到Mycoplasma capricolum受体细胞中.
- 利用聚乙烯糖醇介导的转化用于DNA输送.
- 选择了对四环素耐药性的选择,以确定成功的转化剂.
主要成果:
- 成功创建了含有完整Mycoplasma mycoides LC基因组的细菌细胞.
- 在得到的细胞中消除了可检测的受体基因组序列.
- 移植的基因组赋予了与供体菌株相同的表型.
结论:
- 基因组移植是创建具有合成基因组的细胞的可行方法.
- 这项技术代表了人工基因组的人工传播的重要一步.
- 由此产生的细胞证明了完全更换的基因组的功能可行性.
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