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相关概念视频

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

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相关实验视频

Updated: Jul 3, 2026

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
13:47

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Published on: March 23, 2012

通过非编码RNAs对人类HOX位点中的活性和静态染色体域的功能划分.

John L Rinn1, Michael Kertesz, Jordon K Wang

  • 1Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Cell
|July 3, 2007
PubMed
概括

这项研究在人类HOX位点中确定了231种新的非编码RNA (ncRNA). 一个ncRNA,HOTAIR,通过与Polycomb Repressive Complex 2 (PRC2) 相互作用来抑制远处的HOXD位点的转录.

更多相关视频

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

相关实验视频

Last Updated: Jul 3, 2026

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
13:47

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Published on: March 23, 2012

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

科学领域:

  • 遗传学 是一个遗传学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学

背景情况:

  • 非编码RNAs (ncRNAs) 在表观遗传调节中发挥作用,但它们的功能尚未完全理解.
  • 人类HOX位点及其相关ncRNA的转录景观需要进一步的表征.

研究的目的:

  • 为了全面地绘制人类HOX loci. 的转录景观.
  • 识别和描述HOX loci中的新型ncRNA,并研究它们的调节作用.
  • 探索ncRNAs在基因沉默的染色体域划分中的潜力.

主要方法:

  • 在11个解剖部位的四个人类HOX位点的高分辨率 (五个基对) 转录概况.
  • 新型HOX ncRNAs的识别和表征.
  • 研究ncRNAs与表观遗传修饰剂的相互作用,如Polycomb抑制综合体2 (PRC2).
  • 评估ncRNA表达对基因素甲基化和RNA聚合酶可访问性的影响.

主要成果:

  • 发现231个HOXncRNAs,显著扩展已知的转录区域.
  • HOX ncRNA 沿着发育轴表现出空间表达模式,并具有独特的序列动图.
  • ncRNA表达与差异性基因组甲基化和RNA聚合酶可访问性相关,定义染色体域.
  • 识别HOTAIR,一个在HOXC位点中的2.2kb的ncRNA,抑制在HOXD位点的转录.
  • 霍泰尔与PRC2相互作用,在HOXD位点中调解PRC2占用和H3K27三甲基化.

结论:

  • 对ncRNAs的转录可以划分基因沉默的遥远染色体域.
  • 在HOTAIR中所示的ncRNAs是表观遗传基因沉默的关键调节者.
  • 这些发现对理解发育和疾病中的基因调节有重大影响.